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Identifier
NSP1

Gene
ORF1ab
Protein name
Replicase polyprotein 1ab – Host translation inhibitor nsp1
Localization
unknown
Uniprot
P0DTD1
Description

Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response. (From UniProt)

Information & downloads

Downloads

These downloads are specific to this bait. Full data set downloads can be found on the downloads page.

Sequence

MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGG
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Visualization

Specificity

Plot the specificity of a prey versus its spectral count. The specificty of a prey is calculated as the fold change in the spectral count relative to the average across all other baits. Any other conditions/treatments for the query bait are ignored when calculating the average. Every other bait can contribute a single value when calculting the average, and this value is the maximum spectral count across any conditions present for that bait.

Orange points have infinite specificty. Hover over a point to see its label or click on a point to add a label to the plot.

Select a condition

Options

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Compare conditions

Compare the spectral counts between two different conditions. Any prey passing the option criteria for at least one bait will be displayed. Hover over a point to see its label or click on a point to add a label to the plot.

Select a pair of conditions

Options

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Preys

Condition legend
  • Ct: C-terminal tag
  • H165A: Histidine to alanine mutation at residue 166
  • K164A: Lysine to alanine mutation at residue 164
  • Nt: N-terminal tag
  • SC: spectral counts, calculated as the average spectral count between replicates minus the average detect in controls
  • Spec: specificity, calculated as the fold enrichment of a prey relative to all other bait proteins
  • FDR: false discovery rate
Prey
ID (Entrez / UniProt)ConditionsProfile
C-terminal tag (Ct)K164A-H165A-CtK164A-H165A-NtN-terminal tag (Nt)
S.C.
Spec.
FDR
S.C.
Spec.
FDR
S.C.
Spec.
FDR
S.C.
Spec.
FDR
CCT810694 / P509904509.750.5855211.960132.52.870.62000.62
EIF3A8661 / Q14152266.08283.5307.588.080.558.589.140.55180.08191.890
EIF3G8666 / O7582195.17130.101.171.60.472.172.970.4661.1783.620
PRRC2B84726 / Q5JSZ551.7542.0506.755.480.552.752.230.5619.7516.050.49
POLA15422 / P0988421.6731.850111.17163.390119.67175.8902.173.190.42
PRSS15644 / P0747711.675.170.05---4.171.850.3427.1712.030
RPS106204 / P467837Infinity0------7Infinity0
TANC185461 / Q9C0D55.334.470.52119.83100.57087.3373.290---
RPS206224 / P6086641040------1.5390.07
KPRP448834 / Q5T7493.57.280------24.160.05
SRSF66431 / Q132473.3315.770.01------3.3315.770.01
POLA223649 / Q141811.925.530.3325.9274.71016.4247.330---
ARID1A8289 / O144970.420.280.3411.927.960------
MGA23269 / Q8IWI9000.6298.924.4041.921.860.57---
ECPAS23392 / Q5VYK3---13.9255.5108.4233.580.01---
TXNL19352 / O43396------13.52.160---
ARFGEF210564 / Q9Y6D5---40.837.98025.334.950---
ARFGEF110565 / Q9Y6D6---57.837.57025.333.320---
PRPF810594 / Q6P2Q9---494.920242.410.41---
USP349736 / Q70CQ2---14.583.65018.584.650---
TASOR254906 / Q5VWN6---34.089.560.0114.083.950.49---
IFT7480173 / Q96LB3---18.5160.33016.51430---
IFT8128981 / Q8WYA0---3.59103.5910.04---
CHD857680 / Q9HCK8---18.756.5402.250.790.23---
CHUK1147 / O15111------280---
ARID1B57492 / Q8NFD5---712.5501.52.690.07---
ATRX546 / P46100---4.173.080.011.671.230.17---
POGZ23126 / Q7Z3K3---45.940------
CAD790 / P27708---3.330.330.01------
ZMYND823613 / Q9ULU4---38.670------
AQR9716 / O60306---3.522.750------
ZNF518A9849 / Q6AHZ1---4.5780------
CCDC18655088 / Q7Z3E2---3.57.580------
YEATS255689 / Q9ULM3---15.25260------
CHD684181 / Q8TD26---5.253.570.01------
RPS126206 / P25398---------3Infinity0