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Identifier
NSP3

Gene
ORF1ab
Protein name
Replicase polyprotein 1ab – Non-structural protein 3
Localization
unknown
Uniprot
P0DTD1
Description

Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling. (From UniProt)

Information & downloads

Downloads

These downloads are specific to this bait. Full data set downloads can be found on the downloads page.

Sequence

APTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGG
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Visualization

Specificity

Plot the specificity of a prey versus its spectral count. The specificty of a prey is calculated as the fold change in the spectral count relative to the average across all other baits. Any other conditions/treatments for the query bait are ignored when calculating the average. Every other bait can contribute a single value when calculting the average, and this value is the maximum spectral count across any conditions present for that bait.

Orange points have infinite specificty. Hover over a point to see its label or click on a point to add a label to the plot.

Select a condition

Options

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Compare conditions

Compare the spectral counts between two different conditions. Any prey passing the option criteria for at least one bait will be displayed. Hover over a point to see its label or click on a point to add a label to the plot.

Select a pair of conditions

Options

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Preys

Condition legend
  • C857A: Cysteine to alanine mutation at residue 857
  • Ct: C-terminal tag
  • Nt: N-terminal tag
  • SC: spectral counts, calculated as the average spectral count between replicates minus the average detect in controls
  • Spec: specificity, calculated as the fold enrichment of a prey relative to all other bait proteins
  • FDR: false discovery rate
Prey
ID (Entrez / UniProt)ConditionsProfile
C857A-CtC857A-NtC-terminal tag (Ct)N-terminal tag (Nt)
S.C.
Spec.
FDR
S.C.
Spec.
FDR
S.C.
Spec.
FDR
S.C.
Spec.
FDR
MCCC156922 / Q96RQ3564.335.630570.835.690535.835.350498.834.980
PRSS15644 / P0747722.179.19020.178.360.017.172.970.2123.179.610
ATP2A2488 / P1661513.670.40.01---------
MBOAT779143 / Q96N664.830.490.01------1.330.130.17
POR5447 / P164354.750.350.01---------
AKR1C11645 / Q048283.52.6801.51.150.071.51.150.071.51.150.07
TEX26451368 / Q9Y6I93.50.340---------
SRSF66431 / Q132473.3315.770.010.833.930.183.3315.770.011.336.30.17
ARPC1B10095 / O151432.830.50.023.330.590.01---3.330.590.01
RPL216144 / P467782.50.49030.590---2.50.490
STT3A3703 / P469772.50.420------20.330
DHCR71717 / Q9UBM72.50.4020.320---10.160.1
CYB5A1528 / P001672.50.650---------
RPL306156 / P6288810.290.120.580---20.580
TMEM3355161 / P5708810.220.130.650---10.220.1
FXR18087 / P51114---43.5815.180---41.5814.480
FXR29513 / P51116---24.5810.340---26.5811.180
H1-53009 / P16401---2.838.850.153.3310.420.010.832.60.18
BLMH642 / Q13867---4.5130------
TGM37053 / Q08188---3.337.230.01------
CASP1423581 / P31944---310.40------
KPRP448834 / Q5T749---511.820------
CDSN1041 / Q15517---5200------
ARG1383 / P05089---3.3311.070.01------